2020年11月5日(礼拜四)上午10:00 生科楼2018集会室: DNA replication termination in C. elegans
发布日期: 2020-11-02 阅读次数:   信息来源:瑞典1分彩

农业生物技术国度重点尝试室学术报告

标题问题: DNA replication termination in C. elegans

报告人: Dr. Yisui Xia(夏乙燧) University of Dundee, UK.

时候: 2020115日上午10:00

地址: 性命科学研讨中间2018集会室

接洽人: 楼慧强 传授

报告人简介:

09/2009 – 10/2016 Ph.D. College of Biological Sciences, China Agricultural University

10/2016 – Present Postdoctoral researcher in MRC PPU, Dundee University

 

The core of replisome is replicative helicase CMG complex, which in turn is formed by the six Mcm2-7 ATPases, the Cdc45 protein, and the GINS complex (CMG = Cdc45-MCM-GINS). The CMG complex remains associated tightly with replication forks throughout replication elongation since it can’t be re-assembled. This remarkably stable association of CMG with replication forks implies a particular mechanism is required to unload this helicase and trigger replisome disassembly during DNA replication termination, which also means the disassembly of CMG is the primary step of unloading the replicative machinery from the chromosome when replication is finished.

   CMG disassembly is initiated by ubiquitylation of Mcm7 subunit of hexamer ATPase, leading rapidly to a disassembly reaction that requires Cdc48 (also known as p97) AAA+ ATPase ‘unfoldase’ with its main-adaptors Npl4 and Ufd1.

   I am going to show how CUL-2LRR-1 is regulated during DNA replication termination by using both in vivo and in vitro approaches. Especially, we reconstituted worm CMG ubiquitylation and following step disassembly by purified protein in vitro, which reveals the molecular mechanism of CMG ubiquitylation and disassembly during replication termination.

 

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